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coli expression system and the target gene encoding Pro143-Ser288 is expressed
Then a TMB substrate solution is added to each well
It is suitable for various Taq enzyme-based hot-start PCR and qPCR reactions
Then a biotinylated detection antibody specific for Human BDNF and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated
Mini-sample Mouse RETN (Resistin) ELISA Kit Reagents coli expression system and theThis Resistin ELISA kit uses the Sandwich ELISA principle. The micro ELISA plate provided in this kit has been pre coated with an antibody specific to Mouse RETN. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Mouse RETN and Avidin Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free
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