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Then Streptavidin-HRP is added and binds to the Biotinylated OCM antibody
The OD value is proportional to the concentration of PDGF-BB
MTA1 present in the sample is added and binds to antibodies coated on the wells
Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to CCR2
Human HEPH (Hephaestin) ELISA Kit Packaging:96 wells Then Streptavidin-HRP is added andThe Human HEPH (Hephaestin) ELISA Kit measures HEPH in Human Serum, Plasma, Cell Culture Supernatant, Cell or Tissue Lysate. This kit is based on Double antibody Sandwich ELISA detection method and takes 4h assay time. The microplate provided in this kit has been precoated with anti HEPH antibody. Add standard and properly diluted sample into relevant well respectively. After incubation, wash unbound components. Add biotinylated detection antibody.
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